Using Oral Microbiota Data to Design a Short Sucrose Intake Index

Anders Esberg; Linda Eriksson; Pamela Hasslöf; Simon Haworth; Pernilla Lif Holgerson; Ingegerd Johansson

doi:10.3390/nu13051400

Using Oral Microbiota Data to Design a Short Sucrose Intake Index

Anders Esberg, Linda Eriksson, Pamela Hasslöf, Simon Haworth, Pernilla Lif Holgerson, Ingegerd Johansson

Bristol Dental School

Research output: Contribution to journal › Article (Academic Journal) › peer-review

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Abstract

Excessive sucrose consumption is associated with numerous health problems, including dental caries, and is considered to play a critical role in shaping the human microbiota. Here, we aimed to confirm the association between sucrose exposure and oral microbiota profile, develop a short food-based index capturing variation among sucrose consumers and validate it against oral microbiota and dental caries in a derivation cohort with 16- to 79-year-old participants (n = 427). Intake and food preferences were recorded by questionnaires and saliva microbiota by 16S rDNA sequencing. Taxonomic similarities clustered participants into five clusters, where one stood out with highest sucrose intake and predicted sugar related metabolic pathways but lowest species diversity in the microbiota. Multivariate modelling of food intake and preferences revealed foods suitable for a sucrose index. This, similarly to sucrose intake, was related to bacterial pattern and caries status. The validity of the sucrose index was replicated in the population-based Gene-Lifestyle Interactions in Dental Endpoints (GLIDE, n = 105,520 Swedish adults) cohort. This suggested that the index captured clinically relevant variation in sucrose intake and that FFQ derived information may be suitable for screening of sucrose intake in the clinic and epidemiological studies, although adjustments to local consumption habits are needed.

Original language	English
Article number	1400
Journal	Nutrients
Volume	13
Issue number	5
DOIs	https://doi.org/10.3390/nu13051400
Publication status	Published - 21 Apr 2021

Bibliographical note

Funding Information:
This research was funded by the Swedish Research Council (grant number 2011-03372 and 2015-02597, I.J.), The Swedish Patent Revenue Fund (grant 2017-019, I.J.), and the County Council of V?sterbotten Internal Research Foundation, grant number VLL-831231 (L.E.). The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

Funding Information:
Funding: This research was funded by the Swedish Research Council (grant number 2011-03372 and 2015-02597, I.J.), The Swedish Patent Revenue Fund (grant 2017-019, I.J.), and the County Council of Västerbotten Internal Research Foundation, grant number VLL-831231 (L.E.). The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Adolescent
Adult
Aged
Bacteria/genetics
Cohort Studies
Dental Caries/microbiology
Dietary Sucrose/pharmacology
Female
Humans
Least-Squares Analysis
Male
Microbiota/drug effects
Middle Aged
Mouth/microbiology
Principal Component Analysis
RNA, Ribosomal, 16S/genetics
Statistics, Nonparametric
Young Adult

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/nu13051400

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title = "Using Oral Microbiota Data to Design a Short Sucrose Intake Index",

abstract = "Excessive sucrose consumption is associated with numerous health problems, including dental caries, and is considered to play a critical role in shaping the human microbiota. Here, we aimed to confirm the association between sucrose exposure and oral microbiota profile, develop a short food-based index capturing variation among sucrose consumers and validate it against oral microbiota and dental caries in a derivation cohort with 16- to 79-year-old participants (n = 427). Intake and food preferences were recorded by questionnaires and saliva microbiota by 16S rDNA sequencing. Taxonomic similarities clustered participants into five clusters, where one stood out with highest sucrose intake and predicted sugar related metabolic pathways but lowest species diversity in the microbiota. Multivariate modelling of food intake and preferences revealed foods suitable for a sucrose index. This, similarly to sucrose intake, was related to bacterial pattern and caries status. The validity of the sucrose index was replicated in the population-based Gene-Lifestyle Interactions in Dental Endpoints (GLIDE, n = 105,520 Swedish adults) cohort. This suggested that the index captured clinically relevant variation in sucrose intake and that FFQ derived information may be suitable for screening of sucrose intake in the clinic and epidemiological studies, although adjustments to local consumption habits are needed.",

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author = "Anders Esberg and Linda Eriksson and Pamela Hassl{\"o}f and Simon Haworth and Holgerson, {Pernilla Lif} and Ingegerd Johansson",

note = "Funding Information: This research was funded by the Swedish Research Council (grant number 2011-03372 and 2015-02597, I.J.), The Swedish Patent Revenue Fund (grant 2017-019, I.J.), and the County Council of V?sterbotten Internal Research Foundation, grant number VLL-831231 (L.E.). The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. Funding Information: Funding: This research was funded by the Swedish Research Council (grant number 2011-03372 and 2015-02597, I.J.), The Swedish Patent Revenue Fund (grant 2017-019, I.J.), and the County Council of V{\"a}sterbotten Internal Research Foundation, grant number VLL-831231 (L.E.). The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

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AU - Eriksson, Linda

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AU - Haworth, Simon

AU - Holgerson, Pernilla Lif

AU - Johansson, Ingegerd

N1 - Funding Information: This research was funded by the Swedish Research Council (grant number 2011-03372 and 2015-02597, I.J.), The Swedish Patent Revenue Fund (grant 2017-019, I.J.), and the County Council of V?sterbotten Internal Research Foundation, grant number VLL-831231 (L.E.). The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. Funding Information: Funding: This research was funded by the Swedish Research Council (grant number 2011-03372 and 2015-02597, I.J.), The Swedish Patent Revenue Fund (grant 2017-019, I.J.), and the County Council of Västerbotten Internal Research Foundation, grant number VLL-831231 (L.E.). The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

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N2 - Excessive sucrose consumption is associated with numerous health problems, including dental caries, and is considered to play a critical role in shaping the human microbiota. Here, we aimed to confirm the association between sucrose exposure and oral microbiota profile, develop a short food-based index capturing variation among sucrose consumers and validate it against oral microbiota and dental caries in a derivation cohort with 16- to 79-year-old participants (n = 427). Intake and food preferences were recorded by questionnaires and saliva microbiota by 16S rDNA sequencing. Taxonomic similarities clustered participants into five clusters, where one stood out with highest sucrose intake and predicted sugar related metabolic pathways but lowest species diversity in the microbiota. Multivariate modelling of food intake and preferences revealed foods suitable for a sucrose index. This, similarly to sucrose intake, was related to bacterial pattern and caries status. The validity of the sucrose index was replicated in the population-based Gene-Lifestyle Interactions in Dental Endpoints (GLIDE, n = 105,520 Swedish adults) cohort. This suggested that the index captured clinically relevant variation in sucrose intake and that FFQ derived information may be suitable for screening of sucrose intake in the clinic and epidemiological studies, although adjustments to local consumption habits are needed.

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KW - Adolescent

KW - Adult

KW - Aged

KW - Bacteria/genetics

KW - Cohort Studies

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KW - Dietary Sucrose/pharmacology

KW - Female

KW - Humans

KW - Least-Squares Analysis

KW - Male

KW - Microbiota/drug effects

KW - Middle Aged

KW - Mouth/microbiology

KW - Principal Component Analysis

KW - RNA, Ribosomal, 16S/genetics

KW - Statistics, Nonparametric

KW - Young Adult

U2 - 10.3390/nu13051400

DO - 10.3390/nu13051400

M3 - Article (Academic Journal)

C2 - 33919427

SN - 2072-6643

VL - 13

JO - Nutrients

JF - Nutrients

IS - 5

M1 - 1400

ER -

Using Oral Microbiota Data to Design a Short Sucrose Intake Index

Abstract

Bibliographical note

Keywords

UN SDGs

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