Uukuniemi Phlebovirus assembly and secretion leave a functional imprint on the virion glycome

Max Crispin; David J Harvey; David Bitto; Steinar Halldorsson; Camille Bonomelli; Matthew Edgeworth; James H Scrivens; Juha T Huiskonen; Thomas A Bowden

doi:10.1128/JVI.01662-14

Uukuniemi Phlebovirus assembly and secretion leave a functional imprint on the virion glycome

Max Crispin, David J Harvey, David Bitto, Steinar Halldorsson, Camille Bonomelli, Matthew Edgeworth, James H Scrivens, Juha T Huiskonen, Thomas A Bowden

School of Cellular and Molecular Medicine

Research output: Contribution to journal › Article (Academic Journal) › peer-review

21 Citations (Scopus)

Abstract

Uukuniemi virus (UUKV) is a model system for investigating the genus Phlebovirus of the Bunyaviridae. We report the UUKV glycome, revealing differential processing of the Gn and Gc virion glycoproteins. Both glycoproteins display poly-N-acetyllactosamines, consistent with virion assembly in the medial Golgi apparatus, whereas oligomannose-type glycans required for DC-SIGN-dependent cellular attachment are predominant on Gc. Local virion structure and the route of viral egress from the cell leave a functional imprint on the phleboviral glycome.

Original language	English
Pages (from-to)	10244-51
Number of pages	8
Journal	Journal of Virology
Volume	88
Issue number	17
DOIs	https://doi.org/10.1128/JVI.01662-14
Publication status	Published - 1 Sept 2014

Keywords

Glucans/analysis
Glycomics
Glycoproteins/chemistry
Humans
Uukuniemi virus/physiology
Viral Proteins/chemistry
Virion/chemistry
Virus Assembly
Virus Release

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title = "Uukuniemi Phlebovirus assembly and secretion leave a functional imprint on the virion glycome",

abstract = "Uukuniemi virus (UUKV) is a model system for investigating the genus Phlebovirus of the Bunyaviridae. We report the UUKV glycome, revealing differential processing of the Gn and Gc virion glycoproteins. Both glycoproteins display poly-N-acetyllactosamines, consistent with virion assembly in the medial Golgi apparatus, whereas oligomannose-type glycans required for DC-SIGN-dependent cellular attachment are predominant on Gc. Local virion structure and the route of viral egress from the cell leave a functional imprint on the phleboviral glycome. ",

keywords = "Glucans/analysis, Glycomics, Glycoproteins/chemistry, Humans, Uukuniemi virus/physiology, Viral Proteins/chemistry, Virion/chemistry, Virus Assembly, Virus Release",

author = "Max Crispin and Harvey, {David J} and David Bitto and Steinar Halldorsson and Camille Bonomelli and Matthew Edgeworth and Scrivens, {James H} and Huiskonen, {Juha T} and Bowden, {Thomas A}",

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year = "2014",

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doi = "10.1128/JVI.01662-14",

language = "English",

volume = "88",

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TY - JOUR

T1 - Uukuniemi Phlebovirus assembly and secretion leave a functional imprint on the virion glycome

AU - Crispin, Max

AU - Harvey, David J

AU - Bitto, David

AU - Halldorsson, Steinar

AU - Bonomelli, Camille

AU - Edgeworth, Matthew

AU - Scrivens, James H

AU - Huiskonen, Juha T

AU - Bowden, Thomas A

PY - 2014/9/1

Y1 - 2014/9/1

N2 - Uukuniemi virus (UUKV) is a model system for investigating the genus Phlebovirus of the Bunyaviridae. We report the UUKV glycome, revealing differential processing of the Gn and Gc virion glycoproteins. Both glycoproteins display poly-N-acetyllactosamines, consistent with virion assembly in the medial Golgi apparatus, whereas oligomannose-type glycans required for DC-SIGN-dependent cellular attachment are predominant on Gc. Local virion structure and the route of viral egress from the cell leave a functional imprint on the phleboviral glycome.

AB - Uukuniemi virus (UUKV) is a model system for investigating the genus Phlebovirus of the Bunyaviridae. We report the UUKV glycome, revealing differential processing of the Gn and Gc virion glycoproteins. Both glycoproteins display poly-N-acetyllactosamines, consistent with virion assembly in the medial Golgi apparatus, whereas oligomannose-type glycans required for DC-SIGN-dependent cellular attachment are predominant on Gc. Local virion structure and the route of viral egress from the cell leave a functional imprint on the phleboviral glycome.

KW - Glucans/analysis

KW - Glycomics

KW - Glycoproteins/chemistry

KW - Humans

KW - Uukuniemi virus/physiology

KW - Viral Proteins/chemistry

KW - Virion/chemistry

KW - Virus Assembly

KW - Virus Release

U2 - 10.1128/JVI.01662-14

DO - 10.1128/JVI.01662-14

M3 - Article (Academic Journal)

C2 - 24942574

SN - 0022-538X

VL - 88

SP - 10244

EP - 10251

JO - Journal of Virology

JF - Journal of Virology

IS - 17

ER -

Uukuniemi Phlebovirus assembly and secretion leave a functional imprint on the virion glycome

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Keywords

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