UV-B perceived by the UVR8 photoreceptor inhibits plant thermomorphogenesis

Scott Hayes, Ashutosh Sharma, Donald Fraser, Martine Trevisan, C W Kester Cragg-Barber, Eleni Tavridou, Christian Fankhauser, Gareth Jenkins, Keara Franklin

Research output: Contribution to journalArticle (Academic Journal)

337 Downloads (Pure)

Abstract

Small increases in ambient temperature can elicit striking effects on plant architecture, collectively termed thermomorphogenesis [1]. In Arabidopsis thaliana, these include marked stem elongation and leaf elevation; responses which have been predicted to enhance leaf cooling [2-5]. Thermomorphogenesis requires increased auxin biosynthesis, mediated by the bHLH transcription factor PHYTOCHROME INTERACTING FACTOR 4 (PIF4) [6-8] and enhanced stability of the auxin co-receptor, TIR1, involving HEAT SHOCK PROTEIN 90 (HSP90) [9]. High temperature-mediated hypocotyl elongation additionally involves localised changes in auxin metabolism, mediated by the indole-3-acetic acid (IAA)-amido synthetase Gretchen Hagen 3 (GH3).17 [10]. Here, we show that ultraviolet-B light (UV-B) perceived by the photoreceptor UV RESISTANCE LOCUS8 (UVR8) [11] strongly attenuates thermomorphogenesis via multiple mechanisms inhibiting PIF4 activity. Suppression of thermomorphogenesis involves UVR8 and CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1)-mediated repression of PIF4 transcript accumulation, reducing PIF4 abundance. UV-B also stabilises the bHLH protein, LONG HYPOCOTYL IN FAR RED (HFR1), which can bind to and inhibit PIF4 function. Collectively, our results demonstrate complex crosstalk between UV-B and high temperature signalling. As plants grown in sunlight would likely experience concomitant elevations in UV-B and ambient temperature, elucidating how these pathways are integrated is of key importance to understanding of plant development in natural environments.
Original languageEnglish
Pages (from-to)120-127
Number of pages8
JournalCurrent Biology
Volume27
Issue number1
Early online date15 Dec 2016
DOIs
Publication statusPublished - 9 Jan 2017

    Fingerprint

Cite this

Hayes, S., Sharma, A., Fraser, D., Trevisan, M., Cragg-Barber, C. W. K., Tavridou, E., Fankhauser, C., Jenkins, G., & Franklin, K. (2017). UV-B perceived by the UVR8 photoreceptor inhibits plant thermomorphogenesis. Current Biology, 27(1), 120-127. https://doi.org/10.1016/j.cub.2016.11.004