UVR8 disrupts stabilisation of PIF5 by COP1 to inhibit plant stem elongation in sunlight

Ashutosh Sharma; Bhavana Sharma; Scott Hayes; Konstantin Kerner; Ute Hoecker; Gareth I. Jenkins; Keara A. Franklin

doi:10.1038/s41467-019-12369-1

UVR8 disrupts stabilisation of PIF5 by COP1 to inhibit plant stem elongation in sunlight

Ashutosh Sharma, Bhavana Sharma, Scott Hayes, Konstantin Kerner, Ute Hoecker, Gareth I. Jenkins, Keara A. Franklin^*

^*Corresponding author for this work

Research output: Contribution to journal › Article (Academic Journal) › peer-review

68 Citations (Scopus)

365 Downloads (Pure)

Abstract

Alterations in light quality significantly affect plant growth and development. In canopy shade, phytochrome photoreceptors perceive reduced ratios of red to far-red light (R:FR) and initiate stem elongation to enable plants to overtop competitors. This shade avoidance response is achieved via the stabilisation and activation of PHYTOCHROME INTERACTING FACTORs (PIFs) which elevate auxin biosynthesis. UV-B inhibits shade avoidance by reducing the abundance and activity of PIFs, yet the molecular mechanisms controlling PIF abundance in UV-B are unknown. Here we show that the UV-B photoreceptor UVR8 promotes rapid PIF5 degradation via the ubiquitin-proteasome system in a response requiring the N terminus of PIF5. In planta interactions between UVR8 and PIF5 are not observed. We further demonstrate that PIF5 interacts with the E3 ligase COP1, promoting PIF5 stabilisation in light-grown plants. Binding of UVR8 to COP1 in UV-B disrupts this stabilisation, providing a mechanism to rapidly lower PIF5 abundance in sunlight.

Original language	English
Article number	4417 (2019)
Number of pages	10
Journal	Nature Communications
Volume	10
DOIs	https://doi.org/10.1038/s41467-019-12369-1 https://doi.org/10.1038/s41467-019-12369-1
Publication status	Published - 27 Sept 2019

Keywords

Light responses
Plant development

Access to Document

Full-text PDF (final published version)
This is the final published version of the article (version of record). It first appeared online via Nature at https://doi.org/10.1038/s41467-019-12369-1 . Please refer to any applicable terms of use of the publisher.
Final published version, 1.53 MBLicence: CC BY
Full-text PDF (final published version)
This is the final published version of the article (version of record). It first appeared online via Springer Nature at https://www.nature.com/articles/s41467-019-12369-1. Please refer to any applicable terms of use of the publisher.
Final published version, 1.53 MBLicence: CC BY

Handle.net

Persistent link

Cite this

@article{2f3e528666a44174b187e9117117de87,

title = "UVR8 disrupts stabilisation of PIF5 by COP1 to inhibit plant stem elongation in sunlight",

abstract = "Alterations in light quality significantly affect plant growth and development. In canopy shade, phytochrome photoreceptors perceive reduced ratios of red to far-red light (R:FR) and initiate stem elongation to enable plants to overtop competitors. This shade avoidance response is achieved via the stabilisation and activation of PHYTOCHROME INTERACTING FACTORs (PIFs) which elevate auxin biosynthesis. UV-B inhibits shade avoidance by reducing the abundance and activity of PIFs, yet the molecular mechanisms controlling PIF abundance in UV-B are unknown. Here we show that the UV-B photoreceptor UVR8 promotes rapid PIF5 degradation via the ubiquitin-proteasome system in a response requiring the N terminus of PIF5. In planta interactions between UVR8 and PIF5 are not observed. We further demonstrate that PIF5 interacts with the E3 ligase COP1, promoting PIF5 stabilisation in light-grown plants. Binding of UVR8 to COP1 in UV-B disrupts this stabilisation, providing a mechanism to rapidly lower PIF5 abundance in sunlight.",

keywords = "Light responses, Plant development",

author = "Ashutosh Sharma and Bhavana Sharma and Scott Hayes and Konstantin Kerner and Ute Hoecker and Jenkins, {Gareth I.} and Franklin, {Keara A.}",

year = "2019",

month = sep,

day = "27",

doi = "10.1038/s41467-019-12369-1",

language = "English",

volume = "10",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "Springer Nature",

}

TY - JOUR

T1 - UVR8 disrupts stabilisation of PIF5 by COP1 to inhibit plant stem elongation in sunlight

AU - Sharma, Ashutosh

AU - Sharma, Bhavana

AU - Hayes, Scott

AU - Kerner, Konstantin

AU - Hoecker, Ute

AU - Jenkins, Gareth I.

AU - Franklin, Keara A.

PY - 2019/9/27

Y1 - 2019/9/27

N2 - Alterations in light quality significantly affect plant growth and development. In canopy shade, phytochrome photoreceptors perceive reduced ratios of red to far-red light (R:FR) and initiate stem elongation to enable plants to overtop competitors. This shade avoidance response is achieved via the stabilisation and activation of PHYTOCHROME INTERACTING FACTORs (PIFs) which elevate auxin biosynthesis. UV-B inhibits shade avoidance by reducing the abundance and activity of PIFs, yet the molecular mechanisms controlling PIF abundance in UV-B are unknown. Here we show that the UV-B photoreceptor UVR8 promotes rapid PIF5 degradation via the ubiquitin-proteasome system in a response requiring the N terminus of PIF5. In planta interactions between UVR8 and PIF5 are not observed. We further demonstrate that PIF5 interacts with the E3 ligase COP1, promoting PIF5 stabilisation in light-grown plants. Binding of UVR8 to COP1 in UV-B disrupts this stabilisation, providing a mechanism to rapidly lower PIF5 abundance in sunlight.

AB - Alterations in light quality significantly affect plant growth and development. In canopy shade, phytochrome photoreceptors perceive reduced ratios of red to far-red light (R:FR) and initiate stem elongation to enable plants to overtop competitors. This shade avoidance response is achieved via the stabilisation and activation of PHYTOCHROME INTERACTING FACTORs (PIFs) which elevate auxin biosynthesis. UV-B inhibits shade avoidance by reducing the abundance and activity of PIFs, yet the molecular mechanisms controlling PIF abundance in UV-B are unknown. Here we show that the UV-B photoreceptor UVR8 promotes rapid PIF5 degradation via the ubiquitin-proteasome system in a response requiring the N terminus of PIF5. In planta interactions between UVR8 and PIF5 are not observed. We further demonstrate that PIF5 interacts with the E3 ligase COP1, promoting PIF5 stabilisation in light-grown plants. Binding of UVR8 to COP1 in UV-B disrupts this stabilisation, providing a mechanism to rapidly lower PIF5 abundance in sunlight.

KW - Light responses

KW - Plant development

UR - http://www.scopus.com/inward/record.url?scp=85072729696&partnerID=8YFLogxK

U2 - 10.1038/s41467-019-12369-1

DO - 10.1038/s41467-019-12369-1

M3 - Article (Academic Journal)

C2 - 31562307

AN - SCOPUS:85072729696

SN - 2041-1723

VL - 10

JO - Nature Communications

JF - Nature Communications

M1 - 4417 (2019)

ER -

UVR8 disrupts stabilisation of PIF5 by COP1 to inhibit plant stem elongation in sunlight

Abstract

Keywords

Access to Document

Handle.net

Other files and links

Fingerprint

Integration of UV-B and temperature signalling in plants

Integrating UV-B signalling in to photomorphogenesis networks

Professor Keara A Franklin

Cite this

UVR8 disrupts stabilisation of PIF5 by COP1 to inhibit plant stem elongation in sunlight

Abstract

Keywords

Access to Document

Handle.net

Other files and links

Fingerprint

Projects

Integration of UV-B and temperature signalling in plants

Integrating UV-B signalling in to photomorphogenesis networks

Profiles

Professor Keara A Franklin

Cite this