VEGF-C, VEGF-D and VEGFR-3 expression in peripheral neuroblastic tumours

Pramila Ramani*, Rachel Nash, Lucy Radevsky, Ameesh Patel, Michael Luckett, Chris Rogers

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

6 Citations (Scopus)

Abstract

Ramani P, Nash R, Radevsky L, Patel A, Luckett M & Rogers C ?(2012) Histopathology?VEGF-C, VEGF-D and VEGFR-3 expression in peripheral neuroblastic tumours Aims: More than 50% of neuroblastomas (NBs) present with haematogenous and/or lymphatic metastasis; however, little is known about the clinicopathological significance in NBs of the key lymphangiogenesis growth factors vascular endothelial growth factor (VEGF)-C and VEGF-D and the receptor VEGFR-3. Methods and results: Ninety-three NBs and nine ganglioneuromas (GNs) were immunostained for VEGF-C, VEGF-D and VEGFR-3. VEGF-C and VEGF-D were present in 76% and 82% of the NBs, respectively. There was no significant difference in VEGF-C expression between NBs and GNs. VEGF-D expression was significantly higher in NBs compared with GNs and in MYCN-amplified NBs. VEGFR-3 tumoral cell expression (VEGFR-3c), present in 48% of the NBs, was significantly higher in NBs from children =18 months at presentation and those belonging to a high-risk group. VEGFR-3 lymphovascular density was increased significantly in NBs compared with GNs and in NBs associated with adverse clinicopathological and biological factors. Lymphovascular invasion, assessed in VEGFR-3-stained vessels, was present in similar to 50% of NBs. Cox regression analyses demonstrated that VEGFR-3c expression was associated with a significantly shorter event-free survival and that its effect was independent of the important pathological variable, mitosiskaryorrhexis index. Conclusions: VEGF-D and VEGFR-3 up-regulation support tumour progression in NB and VEGFR-3c may provide a useful prognostic marker in NBs.

Original languageEnglish
Pages (from-to)1006-1016
Number of pages11
JournalHistopathology
Volume61
Issue number6
DOIs
Publication statusPublished - Dec 2012

Structured keywords

  • BTC (Bristol Trials Centre)

Keywords

  • immunohistochemistry
  • FLT4
  • ganglioneuroma
  • VEGF-D
  • VEGF-C
  • ENDOTHELIAL GROWTH-FACTOR
  • BIOLOGICAL FACTORS
  • lymphovascular invasion
  • PATHOLOGY CLASSIFICATION
  • neuroblastoma
  • SOLID TUMORS
  • ANGIOGENIC FACTORS
  • RECEPTOR TYROSINE KINASE
  • DENSITY
  • VEGFR-3
  • LYMPHANGIOGENESIS
  • UP-REGULATION

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