Chromosome segregation is conserved throughout eukaryotes. In most systems, it is solely driven by a spindle machinery that is assembled from microtubules. We have recently discovered that actin filaments that are embedded inside meiotic spindles (spindle actin) are needed for accurate chromosome segregation in mammalian oocytes. To understand the function of spindle actin in oocyte meiosis, we have developed high-resolution and super-resolution live and immunofluorescence microscopy assays that are described in this chapter.
|Title of host publication||Cytoskeleton Dynamics|
|Number of pages||28|
|ISBN (Print)||ISBN 978-1-0716-0218-8, 978-1-0716-0219-5|
|Publication status||E-pub ahead of print - 27 Dec 2019|
|Name||Methods in Molecular Biology|
- High-resolution live microscopy