Visualization and Functional Analysis of Spindle Actin and Chromosome Segregation in Mammalian Oocytes

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

Abstract

Chromosome segregation is conserved throughout eukaryotes. In most systems, it is solely driven by a spindle machinery that is assembled from microtubules. We have recently discovered that actin filaments that are embedded inside meiotic spindles (spindle actin) are needed for accurate chromosome segregation in mammalian oocytes. To understand the function of spindle actin in oocyte meiosis, we have developed high-resolution and super-resolution live and immunofluorescence microscopy assays that are described in this chapter.
Original languageEnglish
Title of host publicationCytoskeleton Dynamics
PublisherHumana Press
Chapter17
Pages267-295
Number of pages28
ISBN (Print)ISBN 978-1-0716-0218-8, 978-1-0716-0219-5
DOIs
Publication statusE-pub ahead of print - 27 Dec 2019

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
ISSN (Print)1064-3745

Keywords

  • Actin
  • Microtubules
  • Chromosomes
  • Meiosis
  • Oocytes
  • Eggs
  • Spindle
  • Fertility
  • High-resolution live microscopy

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