Visualization and quantification of transmembrane ion transport into giant unilamellar vesicles

Hennie Valkenier, Néstor López Mora, Alexander Kros, Anthony P. Davis*

*Corresponding author for this work

Research output: Contribution to journalArticle (Academic Journal)peer-review

27 Citations (Scopus)

Abstract

Transmembrane ion transporters (ionophores) are widely investigated as supramolecular agents with potential for biological activity. Tests are usually performed in synthetic membranes that are assembled into large unilamellar vesicles (LUVs). However transport must be followed through bulk properties of the vesicle suspension, because LUVs are too small for individual study. An alternative approach is described whereby ion transport can be revealed and quantified through direct observation. The method employs giant unilamellar vesicles (GUVs), which are 20-60 mm in diameter and readily imaged by light microscopy. This allows characterization of individual GUVs containing transporter molecules, followed by studies of transport through fluorescence emission from encapsulated indicators. The method provides new levels of certainty and relevance, given that the GUVs are similar in size to living cells. It has been demonstrated using a highly active anion carrier, and should aid the development of compounds for treating channelopathies such as cystic fibrosis.

Original languageEnglish
Pages (from-to)2137-2141
Number of pages5
JournalAngewandte Chemie - International Edition
Volume54
Issue number7
DOIs
Publication statusPublished - 9 Feb 2015

Keywords

  • Anions
  • Giant unilamellar vesicles
  • Ion transport
  • Membranes
  • Supramolecular chemistry

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