Whole-cell patch-clamp recording of voltage-sensitive ca(2+) channel currents in single cells: heterologous expression systems and neurones

Jon Brown, Atticus H Hainsworth, Alessandro Stefani, Andy D Randall

Research output: Contribution to journalArticle (Academic Journal)peer-review

Abstract

Voltage-sensitive calcium channels (VSCC) are vital to the normal physiology of many cell types, including neurones, skeletal, cardiac and smooth muscle cells, heart pacemaker tissue and endocrine cells. Whole-cell recording is a functional electrophysiological assay that allows real-time measurement of macroscopic VSCC activity at the level of single cells. Using this technique, it is possible to probe the molecular physiology, pharmacology, and biophysics of VSCC proteins with a level of precision rarely surpassed in cell biological studies. With best practice voltage-dependent gating behaviors of VSCCs can be interrogated with temporal resolution
Original languageEnglish
Pages (from-to)123-48
Number of pages26
JournalMethods in Molecular Biology
Volume937
DOIs
Publication statusPublished - 2013

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