TY - JOUR
T1 - Whole-cell patch-clamp recording of voltage-sensitive ca(2+) channel currents in single cells
T2 - heterologous expression systems and neurones
AU - Brown, Jon
AU - Hainsworth, Atticus H
AU - Stefani, Alessandro
AU - Randall, Andy D
PY - 2013
Y1 - 2013
N2 - Voltage-sensitive calcium channels (VSCC) are vital to the normal physiology of many cell types, including neurones, skeletal, cardiac and smooth muscle cells, heart pacemaker tissue and endocrine cells. Whole-cell recording is a functional electrophysiological assay that allows real-time measurement of macroscopic VSCC activity at the level of single cells. Using this technique, it is possible to probe the molecular physiology, pharmacology, and biophysics of VSCC proteins with a level of precision rarely surpassed in cell biological studies. With best practice voltage-dependent gating behaviors of VSCCs can be interrogated with temporal resolution
AB - Voltage-sensitive calcium channels (VSCC) are vital to the normal physiology of many cell types, including neurones, skeletal, cardiac and smooth muscle cells, heart pacemaker tissue and endocrine cells. Whole-cell recording is a functional electrophysiological assay that allows real-time measurement of macroscopic VSCC activity at the level of single cells. Using this technique, it is possible to probe the molecular physiology, pharmacology, and biophysics of VSCC proteins with a level of precision rarely surpassed in cell biological studies. With best practice voltage-dependent gating behaviors of VSCCs can be interrogated with temporal resolution
U2 - 10.1007/978-1-62703-086-1_7
DO - 10.1007/978-1-62703-086-1_7
M3 - Article (Academic Journal)
C2 - 23007583
SN - 1940-6029
VL - 937
SP - 123
EP - 148
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -