Wnt and Neuregulin1/ErbB signalling extends 3D culture of hormone responsive mammary organoids

Thierry Jardé, Bethan Lloyd-Lewis, Mairian Thomas, Howard Kendrick, Lorenzo Melchor, Lauriane Bougaret, Peter D Watson, Kenneth Ewan, Matthew J Smalley, Trevor C Dale

Research output: Contribution to journalArticle (Academic Journal)peer-review

84 Downloads (Pure)

Abstract

The development of in vitro culture systems quantitatively and qualitatively recapitulating normal breast biology is key to the understanding of mammary gland biology. Current three-dimensional mammary culture systems have not demonstrated concurrent proliferation and functional differentiation ex vivo in any system for longer than 2 weeks. Here, we identify conditions including Neuregulin1 and R-spondin 1, allowing maintenance and expansion of mammary organoids for 2.5 months in culture. The organoids comprise distinct basal and luminal compartments complete with functional steroid receptors and stem/progenitor cells able to reconstitute a complete mammary gland in vivo. Alternative conditions are also described that promote enrichment of basal cells organized into multiple layers surrounding a keratinous core, reminiscent of structures observed in MMTV-Wnt1 tumours. These conditions comprise a unique tool that should further understanding of normal mammary gland development, the molecular mechanism of hormone action and signalling events whose deregulation leads to breast tumourigenesis.

Original languageEnglish
Article number13207 (2016)
Number of pages15
JournalNature Communications
Volume7
DOIs
Publication statusPublished - 26 Oct 2016

Keywords

  • Animals
  • Female
  • Gene Expression Regulation, Developmental
  • Karyotyping
  • Mammary Glands, Animal/growth & development
  • Mice, Inbred C57BL
  • Microscopy, Confocal
  • Neuregulin-1/genetics
  • Organoids/growth & development
  • Receptor, ErbB-3/genetics
  • Receptor, ErbB-4/genetics
  • Time-Lapse Imaging/methods
  • Tissue Culture Techniques/methods
  • Wnt Signaling Pathway

Fingerprint

Dive into the research topics of 'Wnt and Neuregulin1/ErbB signalling extends 3D culture of hormone responsive mammary organoids'. Together they form a unique fingerprint.

Cite this