Fatty acid construction within the biosynthesis of the polyketide antibiotic mupirocin

Abstract

Key steps of the biosynthesis of the polyketide antibiotic mupirocin have been demonstrated using assays that mimic the biosynthesis in-vitro. Fatty acid construction in mupirocin was shown to proceed using two ketosynthase domains (MmpF and MmpB_KS) and a series of other tailoring domains. The acyl carrier protein has been shown to play a vital role in shuttling the correct intermediates to the active site of each protein.

Date of Award	22 Mar 2022
Original language	English
Awarding Institution	University of Bristol
Supervisor	Matthew P Crump (Supervisor), Christine Willis (Supervisor) & Paul R Race (Supervisor)