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Acute depletion of diacylglycerol from the cis-Golgi affects localised nuclear envelope morphology during mitosis

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)1402-1413
Number of pages12
JournalJournal of Lipid Research
Volume59
Issue number8
Early online date12 Jun 2018
DOIs
DateAccepted/In press - 4 Jun 2018
DateE-pub ahead of print - 12 Jun 2018
DatePublished (current) - Aug 2018

Abstract

Dysregulation of nuclear envelope (NE) assembly results in various cancers; for example, renal and some lung carcinomas ensue due to NE malformation. The NE is a dynamic membrane compartment and its completion during mitosis, is a highly regulated process but the detailed mechanism still remains incompletely understood. Previous studies have found isolated diacylglycerol (DAG) containing vesicles are essential for completing the fusion of NE in non-somatic cells. We investigated the impact of DAG depletion from cis-Golgi in mammalian cells on NE reassembly. Using advanced electron microscopy, we observed an enriched DAG population of vesicles at the vicinity of the NE gaps of telophase mammalian cells. We applied a miniSOG–
C1-domain tag that localized DAG-enriched vesicles at the perinuclear region, which suggested the existence of NE fusogenic vesicles. We quantified the impact of GolgiDAG depletion by measuring the in situ NE rim curvature of the re-forming NE. The rim curvature in these cells was significantly reduced compared with controls, which indicated a localized defect in NE morphology. Our novel results demonstrate the significance of the role of DAG from cis-Golgi for the regulation of NE assembly.

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  • Full-text PDF (accepted author manuscript)

    Rights statement: This is the author accepted manuscript (AAM). The final published version (version of record) is available online via ASBMB at http://www.jlr.org/content/59/8/1402 . Please refer to any applicable terms of use of the publisher.

    Accepted author manuscript, 2.34 MB, PDF document

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