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Concerted functions of Streptococcus gordonii surface proteins PadA and Hsa mediate activation of human platelets and interactions with extracellular matrix

Research output: Contribution to journalArticle

Original languageEnglish
Article numbere12667
Number of pages14
JournalCellular Microbiology
Volume19
Issue number1
Early online date10 Sep 2016
DOIs
DateAccepted/In press - 7 Sep 2016
DateE-pub ahead of print - 10 Sep 2016
DatePublished (current) - 11 Oct 2016

Abstract

A range of Streptococcus bacteria are able to interact with blood platelets to form a thrombus (clot). Streptococcus gordonii is ubiquitous within the human oral cavity and amongst the common pathogens isolated from subjects with infective endocarditis. Two cell surface proteins, Hsa and PadA, in S. gordonii mediate adherence and activation of platelets. In this study we demonstrate that PadA binds activated platelets and that a NGR (Asparagine-Glycine-Arginine) motif within a 657 amino acid residue N-terminal fragment of PadA is responsible for this, together with two other integrin-like recognition motifs RGT and AGD. PadA also acts in concert with Hsa to mediate binding of S. gordonii to cellular fibronectin and vitronectin, and to promote formation of biofilms. Evidence is presented that PadA and Hsa are each reliant on the other's active presentation on the bacterial cell surface, suggesting cooperativity in functions impacting both colonization and pathogenesis.

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    Rights statement: This is the final published version of the article (version of record). It first appeared online via Wiley at http://onlinelibrary.wiley.com/doi/10.1111/cmi.12667/abstract. Please refer to any applicable terms of use of the publisher.

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