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Designer artificial membrane binding proteins direct stem cells to the myocardium

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)7610 - 7618
Number of pages9
JournalChemical Science
Volume2019
Issue number32
DOIs
DateAccepted/In press - 7 Jun 2019
DatePublished (current) - 3 Jul 2019

Abstract

We present a new cell membrane modification methodology where the inherent heart tissue homing properties of the infectious bacteria Streptococcus gordonii are transferred to human stem cells. This is achieved via the rational design of a
chimeric protein-polymer surfactant cell membrane binding construct, comprising the cardiac fibronectin (Fn) binding domain of the bacterial adhesin protein CshA fused to a supercharged protein. Significantly, the protein-polymer
surfactant hybrid spontaneously inserts into the plasma membrane of stem cells without cytotoxicity, instilling the cells with a high affinity for immobilized fibronectin. Moreover, we show that this cell membrane reengineering approach
significantly improves retention and homing of stem cells delivered either intracardially or intravenously to the myocardium in a mouse model.

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    Rights statement: This is the final published version of the article (version of record). It first appeared online via Royal Society of Chemistry at https://pubs.rsc.org/en/content/articlelanding/2019/sc/c9sc02650a#!divAbstract. Please refer to any applicable terms of use of the publisher.

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