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Detailed study of furan fatty acids in total lipids and the cholesteryl ester fraction of fish liver

Research output: Contribution to journalArticle

Original languageEnglish
Pages (from-to)459-468
Number of pages10
JournalFood Analytical Methods
Volume9
Issue number2
Early online date9 Jun 2015
DOIs
DateE-pub ahead of print - 9 Jun 2015
DatePublished (current) - 2016

Abstract

Furan fatty acids (F-acids) are valuable, bioactive minor components in lipids which are characterized by a furan moiety in the acyl chain. Here, we present a gas chromatography/mass spectrometry method operated in the selected ion monitoring mode (GC/MS-SIM) for the thorough determination of F-acid as methyl esters in fish. The method takes advantage of the molecular ion and two characteristic fragment ions and enables the unequivocal detection of >350 saturated, mono- and dienoic F-acids in four GC/MS-SIM runs. This method was applied to the analysis of the methyl esters obtained from the triacylglyceride (TG) and the cholesteryl ester (CE) fraction of ten individual fish liver samples. Up to 23 Facids, including several new or scarcely detected homologues, were detected in the samples. The concentrations of total Facids ranged from 25 to 280 mg/100 g lipids in the TG fractions and <0.11 to 115 mg/100 g lipids in the CE fractions.

    Research areas

  • Fish liver, Furan fatty acids, Gas chromatography, Massspectrometry, Selectedionmonitoring

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