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Engineered basement membranes: from in vivo considerations to cell-based assays

Research output: Contribution to journalReview article

Original languageEnglish
JournalIntegrative biology
Early online date8 Oct 2018
DOIs
DateAccepted/In press - 27 Sep 2018
DateE-pub ahead of print (current) - 8 Oct 2018

Abstract

Improvements in the physiological relevance of cell-based assays have been enabled by the development of various interdisciplinary methods. However, due to their complexity, in vivo structures such as basement membranes (BMs), which regulate the phenotype of adherent cells, are still difficult to mimic in vitro. The reconstruction of a physiologically relevant BM is crucially important to develop cell-based assays with the capacity for drug screening and disease modelling. Here, we review the biophysical and biochemical properties of BMs in vivo and their interactions with neighbouring cells. We discuss the current methods used to mimic BM functions in cell-based assays according to the type of targeted applications. In doing so, we examine the advantages and limitations of each method as well as exploring approaches to improve the physiological relevance of engineered or cell-derived BMs in vitro.

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  • Full-text PDF (accepted author manuscript)

    Rights statement: This is the author accepted manuscript (AAM). The final published version (version of record) is available online via Royal Soc. of Chemistry at https://pubs.rsc.org/en/content/articlehtml/2018/ib/c8ib00138c. Please refer to any applicable terms of use of the publisher.

    Accepted author manuscript, 1.35 MB, PDF document

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