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Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases

Research output: Contribution to journalArticle

  • Kun Huang
  • Fabio Parmeggiani
  • Helene Ledru
  • Kristian Hollingsworth
  • Jordi Mas Pons
  • Andrea Marchesi
  • Peter Both
  • Ashley P. Mattey
  • Edward Pallister
  • Gregory S. Bulmer
  • Jolanda M. van Munster
  • W Bruce Turnbull
  • Carmen Galan
  • Sabine L Flitsch
Original languageEnglish
Pages (from-to)5920-5924
Number of pages5
JournalOrganic and Biomolecular Chemistry
Early online date30 May 2019
DateSubmitted - 19 May 2019
DateAccepted/In press - 29 May 2019
DateE-pub ahead of print (current) - 30 May 2019


Utilising a fast and sensitive screening method based on imidazolium-tagged probes, we report unprecedented reversible activity of bacterial β1,4-galactosyltransferases to catalyse the transgalactosylation from lactose to N-acetylglucosamine to form N-acetyllactosamine in the presence of UDP. The process is demonstrated by the preparative scale synthesis of pNP-β-LacNAc from lactose using β1,4-galactosyltransferase NmLgtB-B as the only biocatalyst.



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