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High-Density SNP Genotyping Array for Hexaploid Wheat and Its Relatives

Research output: Chapter in Book/Report/Conference proceedingChapter in a book

Original languageEnglish
Title of host publicationWheat Biotechnology
Subtitle of host publicationMethods and Protocols
EditorsPrem Bhalla, Mohan Singh
Publisher or commissioning bodyHumana Press
Pages293-306
Number of pages14
Edition1
ISBN (Electronic)9781493973378
ISBN (Print)9781493973354
DOIs
DateE-pub ahead of print - 15 Sep 2017
DatePublished (current) - 11 Oct 2017

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume1679
ISSN (Print)1064-3745

Abstract

A lack of genetic diversity between wheat breeding lines has been recognised as a significant block to future yield increases. Wheat breeding and pre-breeding strategies are increasingly using material from wheat ancestors or wild relatives to re-introduce diversity. Where molecular markers are polymorphic between the host and introgressed material, they may be used to track the size and location of the introgressed material through generations of backcrossing. To generate markers for this purpose, sequence capture targeted re‐sequencing was carried out for a range of wheat varieties, wheat relatives and wheat progenitors. From these sequences a large number of putative SNPs were identified and used to generate the Axiom® Wheat HD array. A selection of varieties representing a selection of elite wheat breeding material, progenitor species and wild relatives were used to validate the array. The procedures used are described here in detail.

    Research areas

  • Array, Genotyping, SNPs, Triticum aestivum, Wheat

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