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MultiBacMam Bimolecular Fluorescence Complementation (BiFC) tool-kit identifies new small-molecule inhibitors of the CDK5-p25 protein-protein interaction (PPI)

Research output: Contribution to journalArticle

  • Itxaso Bellón-Echeverría
  • Jean Philippe Carralot
  • Andrea Araujo Del Rosario
  • Stephanie Kueng
  • Harald Mauser
  • Georg Schmid
  • Ralf Thoma
  • Imre Berger
Original languageEnglish
Article number5083
Number of pages10
JournalScientific Reports
Volume8
DOIs
DateAccepted/In press - 14 Mar 2018
DatePublished (current) - 23 Mar 2018

Abstract

Protein-protein interactions (PPIs) are at the core of virtually all biological processes in cells. Consequently, targeting PPIs is emerging at the forefront of drug discovery. Cellular assays which closely recapitulate native conditions in vivo are instrumental to understand how small molecule drugs can modulate such interactions. We have integrated MultiBacMam, a baculovirus-based mammalian gene delivery tool we developed, with bimolecular fluorescence complementation (BiFC), giving rise to a highly efficient system for assay development, identification and characterization of PPI modulators. We used our system to analyze compounds impacting on CDK5-p25 PPI, which is implicated in numerous diseases including Alzheimer's. We evaluated our tool-kit with the known inhibitor p5T, and we established a mini-screen to identify compounds that modulate this PPI in dose-response experiments. Finally, we discovered several compounds disrupting CDK5-p25 PPI, which had not been identified by other screening or structure-based methods before.

    Research areas

  • High-throughput screening, Kinases , Synthetic Biology

    Structured keywords

  • BrisSynBio
  • Bristol BioDesign Institute

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    Rights statement: This is the final published version of the article (version of record). It first appeared online via NATURE at http://www.nature.com/articles/s41598-018-23516-x . Please refer to any applicable terms of use of the publisher.

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    Licence: CC BY

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