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16S rRNA next generation sequencing analysis shows bacteria in Alzheimer’s post-mortem brain

Research output: Contribution to journalArticle

Original languageEnglish
Article number195
Number of pages13
JournalFrontiers in Aging Neuroscience
Volume9
DOIs
DateIn preparation - 2 Dec 2016
DateAccepted/In press - 31 May 2017
DatePublished (current) - 20 Jun 2017

Abstract

The neurological deterioration associated with Alzheimer’s disease (AD), involving accumulation of amyloid-beta peptides and neurofibrillary tangles, is associated with evident neuroinflammation. This is now seen to be a significant contributor to pathology. Recently the tenet of the privileged status of the brain, regarding microbial compromise, has been questioned, particularly in terms of neurodegenerative diseases. It is now being considered that microbiological incursion into the central nervous system could be either an initiator or significant contributor to these. This is a novel study using 16S ribosomal gene-specific Next generation sequencing (NGS) of extracted brain tissue. A
comparison was made of the bacterial species content of both frozen and formaldehyde fixed sections of a small cohort of Alzheimer-affected cases with those of cognitively unimpaired (normal). Our findings suggest an increase in bacterial populations in Alzheimer brain tissue compared with normal.

    Research areas

  • Alzheimer's disease (AD), Next generation sequencing (NGS), Bacteria, 16S rRNA, microbiome

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    Rights statement: This is the final published version of the article (version of record). It first appeared online via Frontiers Media at http://journal.frontiersin.org/article/10.3389/fnagi.2017.00195/full. Please refer to any applicable terms of use of the publisher.

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